Nidacon PureSperm SpeediKit

Layering a density gradient correctly is difficult and achieving optimal results can be challenging. In addition, a “swim up” process does not remove sperm with abnormal morphology or damaged DNA, and does not separate out bacteria and viruses from the sperm preparation. For this reason, many clinicians in small clinics or private practices are looking for a better, safe, and effective method of preparing sperm for intra-uterine insemination that will improve their results. Enter Speedkit and the UniLayer filtration system.

Recommendations for viscous samples

It can be extremely difficult to obtain good yields of motile sperm from highly viscous semen samples. Nidacon has developed a method that could be helpful to you in the lab. Simply add PureSperm Buffer to the ejaculate, incubate at 37°C for 15-30 minutes and the sample is ready to use. This will give you a much higher proportion of motile sperm.

Protocol:

For sperm preparation from a viscous semen sample:

1. Bring all solutions to room temperature (17 to 27°C).
2. Measure the volume of the semen sample.
3. Dilute 1+2 (1 part PureSperm Buffer and 2 parts semen sample, e.g., 0.5 ml PureSperm Buffer + 1.0 ml semen sample).
4. Incubate at 37°C for 15-30 minutes.
5. Mix using a pipette.
6. Ready for sperm preparation on a density gradient.